Molecular and FISH analysis of 45S rDNA on BAC molecule of Saccharina japonica

IGS is abundant in polymorphism, which widely used the analysis of intraspecific genetic diversity and phylogenetic relationships among geographical populations. In this study, 45S rDNA repeat unit Saccharina japonica was obtained for first time by BAC clone sequencing. The total length S. 8 995 bp, including 5 420 bp 18S-5.8S–25S rDNA, 3 575 (Intergenic Spacer), with GC content 51.4 %. composed a 465 3′-outer transcribed spacer (ETS), an 874 5′-ETS, 2 236 non-transcribed (NTS), 50.1 Fiber-FISH (fiber-fluorescence situ hybridization) distribution units on bacterial artificial chromosome illustrated that each fiber had at least five continuously moniliform hybridization signal points. This study provided new candidate molecular marker detecting polymorphisms japonica. addition, successful fiber-FISH molecule would contribute to construction physical map map-based cloning kelp.